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Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole
To investigate the genotoxic properties of a food‐derived carcinogen, 3‐amino‐1‐methyl‐5H‐pyrido‐[4,3‐b]indole (Trp‐P‐2), we have tested whether Trp‐P‐2 and its metabolically transformed products can induce DNA recombinations. Trp‐P‐2 is a strong mutagen and its activated form, the N‐hydroxylated de...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920750/ https://www.ncbi.nlm.nih.gov/pubmed/7730138 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03033.x |
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author | Hiramoto, Kazuyuki Kanamitsu, Shin‐ichi Negishi, Kazuo Ikeda, Hideo Hayatsu, Hikoya |
author_facet | Hiramoto, Kazuyuki Kanamitsu, Shin‐ichi Negishi, Kazuo Ikeda, Hideo Hayatsu, Hikoya |
author_sort | Hiramoto, Kazuyuki |
collection | PubMed |
description | To investigate the genotoxic properties of a food‐derived carcinogen, 3‐amino‐1‐methyl‐5H‐pyrido‐[4,3‐b]indole (Trp‐P‐2), we have tested whether Trp‐P‐2 and its metabolically transformed products can induce DNA recombinations. Trp‐P‐2 is a strong mutagen and its activated form, the N‐hydroxylated derivative, Trp‐P‐2(NHOH), is known to form DNA adducts and cause DNA chain cleavage. Using a system in which phage λ undergoes recombination inside host Escherichia coli, we have found that Trp‐P‐2(NHOH), but not Trp‐P‐2 itself, can induce recombination. A nitroso derivative of Trp‐P‐2, Trp‐P‐2(NO), which can be reduced intracellularly to form Trp‐P‐2(NHOH), also induced recombination. Active oxygens are implicated in this recombinogenic action, since Trp‐P‐2(NHOH) is known to undergo spontaneous oxidative degradation, generating active oxygen radicals which can cause DNA chain cleavages. 4‐Hydroxyaminoquinoline N‐oxide and phenyl‐hydroxylamine also showed recombinogenic actions in this assay system; hence, it is suspected that aromatic amine‐type carcinogens have this property in common. |
format | Online Article Text |
id | pubmed-5920750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59207502018-05-11 Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole Hiramoto, Kazuyuki Kanamitsu, Shin‐ichi Negishi, Kazuo Ikeda, Hideo Hayatsu, Hikoya Jpn J Cancer Res Article To investigate the genotoxic properties of a food‐derived carcinogen, 3‐amino‐1‐methyl‐5H‐pyrido‐[4,3‐b]indole (Trp‐P‐2), we have tested whether Trp‐P‐2 and its metabolically transformed products can induce DNA recombinations. Trp‐P‐2 is a strong mutagen and its activated form, the N‐hydroxylated derivative, Trp‐P‐2(NHOH), is known to form DNA adducts and cause DNA chain cleavage. Using a system in which phage λ undergoes recombination inside host Escherichia coli, we have found that Trp‐P‐2(NHOH), but not Trp‐P‐2 itself, can induce recombination. A nitroso derivative of Trp‐P‐2, Trp‐P‐2(NO), which can be reduced intracellularly to form Trp‐P‐2(NHOH), also induced recombination. Active oxygens are implicated in this recombinogenic action, since Trp‐P‐2(NHOH) is known to undergo spontaneous oxidative degradation, generating active oxygen radicals which can cause DNA chain cleavages. 4‐Hydroxyaminoquinoline N‐oxide and phenyl‐hydroxylamine also showed recombinogenic actions in this assay system; hence, it is suspected that aromatic amine‐type carcinogens have this property in common. Blackwell Publishing Ltd 1995-02 /pmc/articles/PMC5920750/ /pubmed/7730138 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03033.x Text en |
spellingShingle | Article Hiramoto, Kazuyuki Kanamitsu, Shin‐ichi Negishi, Kazuo Ikeda, Hideo Hayatsu, Hikoya Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole |
title | Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole |
title_full | Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole |
title_fullStr | Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole |
title_full_unstemmed | Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole |
title_short | Induction of DNA Recombination by Activated 3‐Amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole |
title_sort | induction of dna recombination by activated 3‐amino‐1‐methyl‐5h‐pyrido[4,3‐b]indole |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920750/ https://www.ncbi.nlm.nih.gov/pubmed/7730138 http://dx.doi.org/10.1111/j.1349-7006.1995.tb03033.x |
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