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Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer

Evidence indicates that high-grade serous ovarian carcinoma arises from the fallopian tube, rather than ovarian surface epithelium. This is termed the ‘tubal origin’ theory. The aim of the present study was to compare the immunophenotype and gene expression profiling among high-grade serous ovarian...

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Autores principales: Xiang, Li, Rong, Guohua, Zhao, Jing, Wang, Zhenyan, Shi, Fengfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920857/
https://www.ncbi.nlm.nih.gov/pubmed/29731902
http://dx.doi.org/10.3892/ol.2018.8346
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author Xiang, Li
Rong, Guohua
Zhao, Jing
Wang, Zhenyan
Shi, Fengfeng
author_facet Xiang, Li
Rong, Guohua
Zhao, Jing
Wang, Zhenyan
Shi, Fengfeng
author_sort Xiang, Li
collection PubMed
description Evidence indicates that high-grade serous ovarian carcinoma arises from the fallopian tube, rather than ovarian surface epithelium. This is termed the ‘tubal origin’ theory. The aim of the present study was to compare the immunophenotype and gene expression profiling among high-grade serous ovarian carcinoma (HGSOC), fallopian tube epithelium (FTE) and ovarian surface epithelium (OSE) based on tubal origin theory, and identify the differential genes associated with ovarian carcinogenesis. A total of 61 cases of fresh tissue samples including 21 cases of HGSOC, 20 cases of OSE, and 20 cases of FTE were obtained following surgical resection. Immunostaining was performed to detect the expression of PAX8, which has been considered as a potential immunophenotype marker of Müllerian origin. Illumina BeadChip was applied for gene expression profiling. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the differential expression of candidate genes between HGSOC and FTE. The results of the present study demonstrated that PAX8 was highly expressed in HGSOC (19/21, 90.4%) and FTE (20/20, 100%), but not in OSE (3/20, 14.3%). A dendrogram generated by cluster analysis indicated a higher similarity of gene expression profile between HGSOC and FTE than OSE. A total of 2,412 differentially expressed genes were identified (absolute fold change >2) between HGSOC and FTE, including 822 upregulated genes in cancer and 1,590 downregulated genes. S100 calcium binding protein P, Ras-interacting protein 1, Wnt family member 5A, tumor-associated calcium signal transducer 2, Dickkopf Wnt signaling pathway inhibitor 3 and tumor suppressor candidate 3 genes were identified as candidate markers, of which the differential gene expression in HGSOC and FTE was confirmed by RT-qPCR (P<0.05). The results indicate the presence of a greater similarity in the immunophenotype and gene expression profile of HGSOC and FTE, when compared with OSE, which was consistent with the tubal origin theory of HGSOC.
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spelling pubmed-59208572018-05-04 Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer Xiang, Li Rong, Guohua Zhao, Jing Wang, Zhenyan Shi, Fengfeng Oncol Lett Articles Evidence indicates that high-grade serous ovarian carcinoma arises from the fallopian tube, rather than ovarian surface epithelium. This is termed the ‘tubal origin’ theory. The aim of the present study was to compare the immunophenotype and gene expression profiling among high-grade serous ovarian carcinoma (HGSOC), fallopian tube epithelium (FTE) and ovarian surface epithelium (OSE) based on tubal origin theory, and identify the differential genes associated with ovarian carcinogenesis. A total of 61 cases of fresh tissue samples including 21 cases of HGSOC, 20 cases of OSE, and 20 cases of FTE were obtained following surgical resection. Immunostaining was performed to detect the expression of PAX8, which has been considered as a potential immunophenotype marker of Müllerian origin. Illumina BeadChip was applied for gene expression profiling. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the differential expression of candidate genes between HGSOC and FTE. The results of the present study demonstrated that PAX8 was highly expressed in HGSOC (19/21, 90.4%) and FTE (20/20, 100%), but not in OSE (3/20, 14.3%). A dendrogram generated by cluster analysis indicated a higher similarity of gene expression profile between HGSOC and FTE than OSE. A total of 2,412 differentially expressed genes were identified (absolute fold change >2) between HGSOC and FTE, including 822 upregulated genes in cancer and 1,590 downregulated genes. S100 calcium binding protein P, Ras-interacting protein 1, Wnt family member 5A, tumor-associated calcium signal transducer 2, Dickkopf Wnt signaling pathway inhibitor 3 and tumor suppressor candidate 3 genes were identified as candidate markers, of which the differential gene expression in HGSOC and FTE was confirmed by RT-qPCR (P<0.05). The results indicate the presence of a greater similarity in the immunophenotype and gene expression profile of HGSOC and FTE, when compared with OSE, which was consistent with the tubal origin theory of HGSOC. D.A. Spandidos 2018-05 2018-03-26 /pmc/articles/PMC5920857/ /pubmed/29731902 http://dx.doi.org/10.3892/ol.2018.8346 Text en Copyright: © Xiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xiang, Li
Rong, Guohua
Zhao, Jing
Wang, Zhenyan
Shi, Fengfeng
Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
title Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
title_full Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
title_fullStr Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
title_full_unstemmed Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
title_short Identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
title_sort identification of candidate genes associated with tubal origin of high-grade serous ovarian cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920857/
https://www.ncbi.nlm.nih.gov/pubmed/29731902
http://dx.doi.org/10.3892/ol.2018.8346
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