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Features of DNA Oligonucleosomal Fragmentation in Human Tumor Cell Lines and Its Detection by Flow Cytometry: Utility and Limitations

Cultured HL‐60, HeLa S3 and WiDr cells were treated with various doses of ethanol, then subjected to flow cytometry and gel electrophoresis of cellular DNA. On electrophoresis of DNA from HL‐60 cells treated with 0.5 or 1.0 mM ethanol, a ladder pattern was recognized after 3 h. At higher doses of et...

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Detalles Bibliográficos
Autores principales: Kikuchi, Hiroaki, Ujiie, Shigeki, Wakui, Akira, Yokoyama, Akiko, Kanamaru, Ryunosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921250/
https://www.ncbi.nlm.nih.gov/pubmed/9045897
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00302.x