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P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
A high incidence of gene mutations or deletions of P16INK4, a cell cycle regulator which inhibits the activity of cyclin‐dependent kinase 4/cyclin D complex and blocks the Gl‐to‐S transition, has been reported in pancreato‐biliary tract cancers. In order to investigateP16INK4 gene alterations in spo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921281/ https://www.ncbi.nlm.nih.gov/pubmed/9414654 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00312.x |
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author | Imai, Yasuo Tsurutani, Naomi Oda, Hideaki Nakatsuru, Yoko Inoue, Tohru Ishikawa, Takatoshi |
author_facet | Imai, Yasuo Tsurutani, Naomi Oda, Hideaki Nakatsuru, Yoko Inoue, Tohru Ishikawa, Takatoshi |
author_sort | Imai, Yasuo |
collection | PubMed |
description | A high incidence of gene mutations or deletions of P16INK4, a cell cycle regulator which inhibits the activity of cyclin‐dependent kinase 4/cyclin D complex and blocks the Gl‐to‐S transition, has been reported in pancreato‐biliary tract cancers. In order to investigateP16INK4 gene alterations in sporadic ampullary carcinomas, 17 sporadic ampullary carcinomas were examined. After histological diagnosis, DNA samples extracted separately from both cancerous and normal paraffin‐embedded tissues were investigated. Loss of heterozygosity (LOH) was investigated utilizing 3 microsatellite markers on 9p21‐22, and a mutationsl analysis was performed by cloning and sequencing. LOH was observed in 3 cases (17.6%) and somatic mutations with retention of heterozygosity were found in 7 cases (41.2%). Of note was that two mutations resulted in truncated incomplete proteins and one was a point mutation at the consensus site in the conserved ankyrin repeats, which would be crucial for function. Although two‐hit inactivation was not evident in any of the mutation cases and further investigation would be needed to elucidate the role of altered p16INK4, these results suggest that the pl6INK4 gene mutations are relatively frequent and its inactivation might be important in ampnllary carcinogenesis. |
format | Online Article Text |
id | pubmed-5921281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59212812018-05-11 P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas Imai, Yasuo Tsurutani, Naomi Oda, Hideaki Nakatsuru, Yoko Inoue, Tohru Ishikawa, Takatoshi Jpn J Cancer Res Article A high incidence of gene mutations or deletions of P16INK4, a cell cycle regulator which inhibits the activity of cyclin‐dependent kinase 4/cyclin D complex and blocks the Gl‐to‐S transition, has been reported in pancreato‐biliary tract cancers. In order to investigateP16INK4 gene alterations in sporadic ampullary carcinomas, 17 sporadic ampullary carcinomas were examined. After histological diagnosis, DNA samples extracted separately from both cancerous and normal paraffin‐embedded tissues were investigated. Loss of heterozygosity (LOH) was investigated utilizing 3 microsatellite markers on 9p21‐22, and a mutationsl analysis was performed by cloning and sequencing. LOH was observed in 3 cases (17.6%) and somatic mutations with retention of heterozygosity were found in 7 cases (41.2%). Of note was that two mutations resulted in truncated incomplete proteins and one was a point mutation at the consensus site in the conserved ankyrin repeats, which would be crucial for function. Although two‐hit inactivation was not evident in any of the mutation cases and further investigation would be needed to elucidate the role of altered p16INK4, these results suggest that the pl6INK4 gene mutations are relatively frequent and its inactivation might be important in ampnllary carcinogenesis. Blackwell Publishing Ltd 1997-10 /pmc/articles/PMC5921281/ /pubmed/9414654 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00312.x Text en |
spellingShingle | Article Imai, Yasuo Tsurutani, Naomi Oda, Hideaki Nakatsuru, Yoko Inoue, Tohru Ishikawa, Takatoshi P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas |
title | P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas |
title_full | P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas |
title_fullStr | P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas |
title_full_unstemmed | P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas |
title_short | P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas |
title_sort | p16ink4gene mutations are relatively frequent in ampullary carcinomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921281/ https://www.ncbi.nlm.nih.gov/pubmed/9414654 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00312.x |
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