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P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas

A high incidence of gene mutations or deletions of P16INK4, a cell cycle regulator which inhibits the activity of cyclin‐dependent kinase 4/cyclin D complex and blocks the Gl‐to‐S transition, has been reported in pancreato‐biliary tract cancers. In order to investigateP16INK4 gene alterations in spo...

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Autores principales: Imai, Yasuo, Tsurutani, Naomi, Oda, Hideaki, Nakatsuru, Yoko, Inoue, Tohru, Ishikawa, Takatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921281/
https://www.ncbi.nlm.nih.gov/pubmed/9414654
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00312.x
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author Imai, Yasuo
Tsurutani, Naomi
Oda, Hideaki
Nakatsuru, Yoko
Inoue, Tohru
Ishikawa, Takatoshi
author_facet Imai, Yasuo
Tsurutani, Naomi
Oda, Hideaki
Nakatsuru, Yoko
Inoue, Tohru
Ishikawa, Takatoshi
author_sort Imai, Yasuo
collection PubMed
description A high incidence of gene mutations or deletions of P16INK4, a cell cycle regulator which inhibits the activity of cyclin‐dependent kinase 4/cyclin D complex and blocks the Gl‐to‐S transition, has been reported in pancreato‐biliary tract cancers. In order to investigateP16INK4 gene alterations in sporadic ampullary carcinomas, 17 sporadic ampullary carcinomas were examined. After histological diagnosis, DNA samples extracted separately from both cancerous and normal paraffin‐embedded tissues were investigated. Loss of heterozygosity (LOH) was investigated utilizing 3 microsatellite markers on 9p21‐22, and a mutationsl analysis was performed by cloning and sequencing. LOH was observed in 3 cases (17.6%) and somatic mutations with retention of heterozygosity were found in 7 cases (41.2%). Of note was that two mutations resulted in truncated incomplete proteins and one was a point mutation at the consensus site in the conserved ankyrin repeats, which would be crucial for function. Although two‐hit inactivation was not evident in any of the mutation cases and further investigation would be needed to elucidate the role of altered p16INK4, these results suggest that the pl6INK4 gene mutations are relatively frequent and its inactivation might be important in ampnllary carcinogenesis.
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spelling pubmed-59212812018-05-11 P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas Imai, Yasuo Tsurutani, Naomi Oda, Hideaki Nakatsuru, Yoko Inoue, Tohru Ishikawa, Takatoshi Jpn J Cancer Res Article A high incidence of gene mutations or deletions of P16INK4, a cell cycle regulator which inhibits the activity of cyclin‐dependent kinase 4/cyclin D complex and blocks the Gl‐to‐S transition, has been reported in pancreato‐biliary tract cancers. In order to investigateP16INK4 gene alterations in sporadic ampullary carcinomas, 17 sporadic ampullary carcinomas were examined. After histological diagnosis, DNA samples extracted separately from both cancerous and normal paraffin‐embedded tissues were investigated. Loss of heterozygosity (LOH) was investigated utilizing 3 microsatellite markers on 9p21‐22, and a mutationsl analysis was performed by cloning and sequencing. LOH was observed in 3 cases (17.6%) and somatic mutations with retention of heterozygosity were found in 7 cases (41.2%). Of note was that two mutations resulted in truncated incomplete proteins and one was a point mutation at the consensus site in the conserved ankyrin repeats, which would be crucial for function. Although two‐hit inactivation was not evident in any of the mutation cases and further investigation would be needed to elucidate the role of altered p16INK4, these results suggest that the pl6INK4 gene mutations are relatively frequent and its inactivation might be important in ampnllary carcinogenesis. Blackwell Publishing Ltd 1997-10 /pmc/articles/PMC5921281/ /pubmed/9414654 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00312.x Text en
spellingShingle Article
Imai, Yasuo
Tsurutani, Naomi
Oda, Hideaki
Nakatsuru, Yoko
Inoue, Tohru
Ishikawa, Takatoshi
P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
title P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
title_full P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
title_fullStr P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
title_full_unstemmed P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
title_short P16INK4Gene Mutations Are Relatively Frequent in Ampullary Carcinomas
title_sort p16ink4gene mutations are relatively frequent in ampullary carcinomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921281/
https://www.ncbi.nlm.nih.gov/pubmed/9414654
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00312.x
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