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N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model

We investigated the effects of N(G)‐nitro‐L‐arginine‐methyI ester (L‐NAME), a nitric oxide synthase (NOS) inhibitor, on hone metastasis of human breast cancer, MDA‐231 cells. Tumor cells (2 × 10(5) cells in 0.2 ml of phosphate‐buffered saline; PBS) were injected through the diaphragm into the left v...

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Autores principales: Iwasaki, Teruo, Higashiyama, Masahiko, Kuriyama, Keiko, Sasaki, Akira, Mukai, Mutsuko, Shinkai, Kiyoko, Horai, Takeshi, Matsuda, Hikaru, Akedo, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921518/
https://www.ncbi.nlm.nih.gov/pubmed/9369934
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00462.x
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author Iwasaki, Teruo
Higashiyama, Masahiko
Kuriyama, Keiko
Sasaki, Akira
Mukai, Mutsuko
Shinkai, Kiyoko
Horai, Takeshi
Matsuda, Hikaru
Akedo, Hitoshi
author_facet Iwasaki, Teruo
Higashiyama, Masahiko
Kuriyama, Keiko
Sasaki, Akira
Mukai, Mutsuko
Shinkai, Kiyoko
Horai, Takeshi
Matsuda, Hikaru
Akedo, Hitoshi
author_sort Iwasaki, Teruo
collection PubMed
description We investigated the effects of N(G)‐nitro‐L‐arginine‐methyI ester (L‐NAME), a nitric oxide synthase (NOS) inhibitor, on hone metastasis of human breast cancer, MDA‐231 cells. Tumor cells (2 × 10(5) cells in 0.2 ml of phosphate‐buffered saline; PBS) were injected through the diaphragm into the left ventricle of the heart of laparotomized nude mice (male 5‐week‐old ICR‐nu/nu). L‐NAME (2 mg/ mouse/injection in 0.1 ml of PBS) was given intraperitoneally to mice 6 h and 3 h before and immediately, 3 h, 6 h, 18 h and 21 h after the intraeardlac injection of tumor cells. As a control, 0.1 ml of PBS was injected instead of L‐NAME. The effect of N(G)‐nitro‐D‐arginine‐methyl ester CD‐NAME; 2 mg/mouse/injection), an inactive analogue of L‐NAME, was also investigated to evaluate the specificity of L‐NAME action. Radiographical examination 31 days after the tumor‐cell injection showed that the incidence and number of osteolytic bone metastases and the number of bones with metastasis in L‐NAME‐treated mice were significantly reduced compared with those in PBS‐treated mice (P<0,05). The differences between PBS‐treated and D‐NAME‐treated mice were not significant. Our findings suggest that specific and appropriate NOS inhibitors may represent a new pharmacological approach to therapy for cancer patients at risk of developing osteolytic bone metastases.
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spelling pubmed-59215182018-05-11 N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model Iwasaki, Teruo Higashiyama, Masahiko Kuriyama, Keiko Sasaki, Akira Mukai, Mutsuko Shinkai, Kiyoko Horai, Takeshi Matsuda, Hikaru Akedo, Hitoshi Jpn J Cancer Res Article We investigated the effects of N(G)‐nitro‐L‐arginine‐methyI ester (L‐NAME), a nitric oxide synthase (NOS) inhibitor, on hone metastasis of human breast cancer, MDA‐231 cells. Tumor cells (2 × 10(5) cells in 0.2 ml of phosphate‐buffered saline; PBS) were injected through the diaphragm into the left ventricle of the heart of laparotomized nude mice (male 5‐week‐old ICR‐nu/nu). L‐NAME (2 mg/ mouse/injection in 0.1 ml of PBS) was given intraperitoneally to mice 6 h and 3 h before and immediately, 3 h, 6 h, 18 h and 21 h after the intraeardlac injection of tumor cells. As a control, 0.1 ml of PBS was injected instead of L‐NAME. The effect of N(G)‐nitro‐D‐arginine‐methyl ester CD‐NAME; 2 mg/mouse/injection), an inactive analogue of L‐NAME, was also investigated to evaluate the specificity of L‐NAME action. Radiographical examination 31 days after the tumor‐cell injection showed that the incidence and number of osteolytic bone metastases and the number of bones with metastasis in L‐NAME‐treated mice were significantly reduced compared with those in PBS‐treated mice (P<0,05). The differences between PBS‐treated and D‐NAME‐treated mice were not significant. Our findings suggest that specific and appropriate NOS inhibitors may represent a new pharmacological approach to therapy for cancer patients at risk of developing osteolytic bone metastases. Blackwell Publishing Ltd 1997-09 /pmc/articles/PMC5921518/ /pubmed/9369934 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00462.x Text en
spellingShingle Article
Iwasaki, Teruo
Higashiyama, Masahiko
Kuriyama, Keiko
Sasaki, Akira
Mukai, Mutsuko
Shinkai, Kiyoko
Horai, Takeshi
Matsuda, Hikaru
Akedo, Hitoshi
N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
title N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
title_full N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
title_fullStr N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
title_full_unstemmed N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
title_short N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
title_sort n(g)‐nitro‐l‐arginine methyl ester inhibits bone metastasis after modified intracardiac injection of human breast cancer cells in a nude mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921518/
https://www.ncbi.nlm.nih.gov/pubmed/9369934
http://dx.doi.org/10.1111/j.1349-7006.1997.tb00462.x
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