Cargando…
N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model
We investigated the effects of N(G)‐nitro‐L‐arginine‐methyI ester (L‐NAME), a nitric oxide synthase (NOS) inhibitor, on hone metastasis of human breast cancer, MDA‐231 cells. Tumor cells (2 × 10(5) cells in 0.2 ml of phosphate‐buffered saline; PBS) were injected through the diaphragm into the left v...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1997
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921518/ https://www.ncbi.nlm.nih.gov/pubmed/9369934 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00462.x |
_version_ | 1783318029830979584 |
---|---|
author | Iwasaki, Teruo Higashiyama, Masahiko Kuriyama, Keiko Sasaki, Akira Mukai, Mutsuko Shinkai, Kiyoko Horai, Takeshi Matsuda, Hikaru Akedo, Hitoshi |
author_facet | Iwasaki, Teruo Higashiyama, Masahiko Kuriyama, Keiko Sasaki, Akira Mukai, Mutsuko Shinkai, Kiyoko Horai, Takeshi Matsuda, Hikaru Akedo, Hitoshi |
author_sort | Iwasaki, Teruo |
collection | PubMed |
description | We investigated the effects of N(G)‐nitro‐L‐arginine‐methyI ester (L‐NAME), a nitric oxide synthase (NOS) inhibitor, on hone metastasis of human breast cancer, MDA‐231 cells. Tumor cells (2 × 10(5) cells in 0.2 ml of phosphate‐buffered saline; PBS) were injected through the diaphragm into the left ventricle of the heart of laparotomized nude mice (male 5‐week‐old ICR‐nu/nu). L‐NAME (2 mg/ mouse/injection in 0.1 ml of PBS) was given intraperitoneally to mice 6 h and 3 h before and immediately, 3 h, 6 h, 18 h and 21 h after the intraeardlac injection of tumor cells. As a control, 0.1 ml of PBS was injected instead of L‐NAME. The effect of N(G)‐nitro‐D‐arginine‐methyl ester CD‐NAME; 2 mg/mouse/injection), an inactive analogue of L‐NAME, was also investigated to evaluate the specificity of L‐NAME action. Radiographical examination 31 days after the tumor‐cell injection showed that the incidence and number of osteolytic bone metastases and the number of bones with metastasis in L‐NAME‐treated mice were significantly reduced compared with those in PBS‐treated mice (P<0,05). The differences between PBS‐treated and D‐NAME‐treated mice were not significant. Our findings suggest that specific and appropriate NOS inhibitors may represent a new pharmacological approach to therapy for cancer patients at risk of developing osteolytic bone metastases. |
format | Online Article Text |
id | pubmed-5921518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59215182018-05-11 N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model Iwasaki, Teruo Higashiyama, Masahiko Kuriyama, Keiko Sasaki, Akira Mukai, Mutsuko Shinkai, Kiyoko Horai, Takeshi Matsuda, Hikaru Akedo, Hitoshi Jpn J Cancer Res Article We investigated the effects of N(G)‐nitro‐L‐arginine‐methyI ester (L‐NAME), a nitric oxide synthase (NOS) inhibitor, on hone metastasis of human breast cancer, MDA‐231 cells. Tumor cells (2 × 10(5) cells in 0.2 ml of phosphate‐buffered saline; PBS) were injected through the diaphragm into the left ventricle of the heart of laparotomized nude mice (male 5‐week‐old ICR‐nu/nu). L‐NAME (2 mg/ mouse/injection in 0.1 ml of PBS) was given intraperitoneally to mice 6 h and 3 h before and immediately, 3 h, 6 h, 18 h and 21 h after the intraeardlac injection of tumor cells. As a control, 0.1 ml of PBS was injected instead of L‐NAME. The effect of N(G)‐nitro‐D‐arginine‐methyl ester CD‐NAME; 2 mg/mouse/injection), an inactive analogue of L‐NAME, was also investigated to evaluate the specificity of L‐NAME action. Radiographical examination 31 days after the tumor‐cell injection showed that the incidence and number of osteolytic bone metastases and the number of bones with metastasis in L‐NAME‐treated mice were significantly reduced compared with those in PBS‐treated mice (P<0,05). The differences between PBS‐treated and D‐NAME‐treated mice were not significant. Our findings suggest that specific and appropriate NOS inhibitors may represent a new pharmacological approach to therapy for cancer patients at risk of developing osteolytic bone metastases. Blackwell Publishing Ltd 1997-09 /pmc/articles/PMC5921518/ /pubmed/9369934 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00462.x Text en |
spellingShingle | Article Iwasaki, Teruo Higashiyama, Masahiko Kuriyama, Keiko Sasaki, Akira Mukai, Mutsuko Shinkai, Kiyoko Horai, Takeshi Matsuda, Hikaru Akedo, Hitoshi N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model |
title | N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model |
title_full | N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model |
title_fullStr | N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model |
title_full_unstemmed | N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model |
title_short | N(G)‐Nitro‐L‐arginine Methyl Ester Inhibits Bone Metastasis after Modified Intracardiac Injection of Human Breast Cancer Cells in a Nude Mouse Model |
title_sort | n(g)‐nitro‐l‐arginine methyl ester inhibits bone metastasis after modified intracardiac injection of human breast cancer cells in a nude mouse model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921518/ https://www.ncbi.nlm.nih.gov/pubmed/9369934 http://dx.doi.org/10.1111/j.1349-7006.1997.tb00462.x |
work_keys_str_mv | AT iwasakiteruo ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT higashiyamamasahiko ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT kuriyamakeiko ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT sasakiakira ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT mukaimutsuko ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT shinkaikiyoko ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT horaitakeshi ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT matsudahikaru ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel AT akedohitoshi ngnitrolargininemethylesterinhibitsbonemetastasisaftermodifiedintracardiacinjectionofhumanbreastcancercellsinanudemousemodel |