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Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells
Vinblastine arrests cells in the G2/M phase of the cell cycle and subsequently induces cell death by apoptosis. We found that treatment of cells with vinblastine induced phosphorylation of Bcl‐2, resulting in the dissociation of Bcl‐2 and Bax. Moreover, vinblastine‐induced apoptosis was suppressed b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921943/ https://www.ncbi.nlm.nih.gov/pubmed/9818030 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00652.x |
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author | Tashiro, Etsu Simizu, Siro Takada, Minoru Umezawa, Kazuo Imoto, Masaya |
author_facet | Tashiro, Etsu Simizu, Siro Takada, Minoru Umezawa, Kazuo Imoto, Masaya |
author_sort | Tashiro, Etsu |
collection | PubMed |
description | Vinblastine arrests cells in the G2/M phase of the cell cycle and subsequently induces cell death by apoptosis. We found that treatment of cells with vinblastine induced phosphorylation of Bcl‐2, resulting in the dissociation of Bcl‐2 and Bax. Moreover, vinblastine‐induced apoptosis was suppressed by an inhibitor of caspase‐3, Ac‐DEVD‐CHO; and a 17‐kDa active fragment of caspase‐3 was detected following vinblastine treatment, suggesting that caspase‐3 is involved in vinblastine‐induced apoptosis. However, Ac‐DEVD‐CHO affected neither vinblastine‐induced Bcl‐2 phosphorylation nor vinblastine‐induced G2/M arrest. Vinblastine caused G2/M arrest prior to apoptosis, whereas vinblastine‐induced apoptosis was not dependent on the duration of the G2/M phase. Thus, vinblastine‐induced apoptosis might be mediated by the phosphorylation of Bcl‐2, resulting in Bcl‐2 inactivation, and by subsequent activation of caspase‐3. |
format | Online Article Text |
id | pubmed-5921943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59219432018-05-11 Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells Tashiro, Etsu Simizu, Siro Takada, Minoru Umezawa, Kazuo Imoto, Masaya Jpn J Cancer Res Article Vinblastine arrests cells in the G2/M phase of the cell cycle and subsequently induces cell death by apoptosis. We found that treatment of cells with vinblastine induced phosphorylation of Bcl‐2, resulting in the dissociation of Bcl‐2 and Bax. Moreover, vinblastine‐induced apoptosis was suppressed by an inhibitor of caspase‐3, Ac‐DEVD‐CHO; and a 17‐kDa active fragment of caspase‐3 was detected following vinblastine treatment, suggesting that caspase‐3 is involved in vinblastine‐induced apoptosis. However, Ac‐DEVD‐CHO affected neither vinblastine‐induced Bcl‐2 phosphorylation nor vinblastine‐induced G2/M arrest. Vinblastine caused G2/M arrest prior to apoptosis, whereas vinblastine‐induced apoptosis was not dependent on the duration of the G2/M phase. Thus, vinblastine‐induced apoptosis might be mediated by the phosphorylation of Bcl‐2, resulting in Bcl‐2 inactivation, and by subsequent activation of caspase‐3. Blackwell Publishing Ltd 1998-09 /pmc/articles/PMC5921943/ /pubmed/9818030 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00652.x Text en |
spellingShingle | Article Tashiro, Etsu Simizu, Siro Takada, Minoru Umezawa, Kazuo Imoto, Masaya Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells |
title | Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells |
title_full | Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells |
title_fullStr | Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells |
title_full_unstemmed | Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells |
title_short | Caspase‐3 Activation Is Not Responsible for Vinblastine‐induced Bcl‐2 Phosphorylation and G2/M Arrest in Human Small Cell Lung Carcinoma Ms‐1 Cells |
title_sort | caspase‐3 activation is not responsible for vinblastine‐induced bcl‐2 phosphorylation and g2/m arrest in human small cell lung carcinoma ms‐1 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921943/ https://www.ncbi.nlm.nih.gov/pubmed/9818030 http://dx.doi.org/10.1111/j.1349-7006.1998.tb00652.x |
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