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Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method t...

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Detalles Bibliográficos
Autores principales: Devulapally, Praneeth Reddy, Bürger, Jörg, Mielke, Thorsten, Konthur, Zoltán, Lehrach, Hans, Yaspo, Marie-Laure, Glökler, Jörn, Warnatz, Hans-Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921987/
https://www.ncbi.nlm.nih.gov/pubmed/29703216
http://dx.doi.org/10.1186/s13073-018-0542-5