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Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes
Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5921987/ https://www.ncbi.nlm.nih.gov/pubmed/29703216 http://dx.doi.org/10.1186/s13073-018-0542-5 |