One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered v...

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Detalles Bibliográficos
Autores principales: Breunig, Christopher T., Durovic, Tamara, Neuner, Andrea M., Baumann, Valentin, Wiesbeck, Maximilian F., Köferle, Anna, Götz, Magdalena, Ninkovic, Jovica, Stricker, Stefan H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922533/
https://www.ncbi.nlm.nih.gov/pubmed/29702666
http://dx.doi.org/10.1371/journal.pone.0196015

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5922533/
https://www.ncbi.nlm.nih.gov/pubmed/29702666
http://dx.doi.org/10.1371/journal.pone.0196015

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