CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR-Cas9 nuclease off-targets

Sensitive detection of off-target effects is important for translating CRISPR-Cas9 nucleases into human therapeutics. In vitro biochemical methods for finding off-targets offer potential advantages of greater reproducibility and scalability while avoiding limitations associated with strategies that...

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Detalles Bibliográficos
Autores principales: Tsai, Shengdar Q., Nguyen, Nhu T., Malagon-Lopez, Jose, Topkar, Ved V., Aryee, Martin J., Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924695/
https://www.ncbi.nlm.nih.gov/pubmed/28459458
http://dx.doi.org/10.1038/nmeth.4278

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5924695/
https://www.ncbi.nlm.nih.gov/pubmed/28459458
http://dx.doi.org/10.1038/nmeth.4278

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