Polymerase Chain Reaction with Confronting Two‐pair Primers for Polymorphism Genotyping

A novel PCR method using confronting two‐pair primers, named PCR‐CTPP, is introduced to detect a single nucleotide polymorphism (base X or Y). One primer for the X allele is set to include X'at the 3′end (antisense), where X'is the antisense of X, with the counterpart sense primer upstream...

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Detalles Bibliográficos
Autores principales: Hamajima, Nobuyuki, Saito, Toshiko, Matsuo, Keitaro, Kozaki, Ken‐ichi, Takahashi, Takashi, Tajima, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2000
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926438/
https://www.ncbi.nlm.nih.gov/pubmed/11011111
http://dx.doi.org/10.1111/j.1349-7006.2000.tb01026.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926438/
https://www.ncbi.nlm.nih.gov/pubmed/11011111
http://dx.doi.org/10.1111/j.1349-7006.2000.tb01026.x

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