The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche

Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, pla...

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Autores principales: Simon, Melinda, Major, Bálint, Vácz, Gabriella, Kuten, Olga, Hornyák, István, Hinsenkamp, Adél, Kardos, Dorottya, Bagó, Marcell, Cseh, Domonkos, Sárközi, Adrienn, Horvathy, Denes, Nehrer, Stefan, Lacza, Zsombor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926480/
https://www.ncbi.nlm.nih.gov/pubmed/29760725
http://dx.doi.org/10.1155/2018/4854619
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author Simon, Melinda
Major, Bálint
Vácz, Gabriella
Kuten, Olga
Hornyák, István
Hinsenkamp, Adél
Kardos, Dorottya
Bagó, Marcell
Cseh, Domonkos
Sárközi, Adrienn
Horvathy, Denes
Nehrer, Stefan
Lacza, Zsombor
author_facet Simon, Melinda
Major, Bálint
Vácz, Gabriella
Kuten, Olga
Hornyák, István
Hinsenkamp, Adél
Kardos, Dorottya
Bagó, Marcell
Cseh, Domonkos
Sárközi, Adrienn
Horvathy, Denes
Nehrer, Stefan
Lacza, Zsombor
author_sort Simon, Melinda
collection PubMed
description Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as “proliferative agents” for the topical MSC pool. Hyperacute serum (HAS), a new serum derivative, has been designed to approximate the natural coagulation cascade with a single-step, additive-free preparation method. We investigated the effects of HAS on monolayer MSC cultures and in their natural niche, in 3D subchondral bone and marrow explants. Viability measurements, RT-qPCR evaluation for gene expression and flow cytometry for cell surface marker analysis were performed to compare the effects of FCS-, PRP-, or HAS-supplemented culture media. Monolayer MSCs showed significantly higher metabolic activity following 5 days' incubation in HAS, and osteoblast-specific mRNA expression was markedly increased, while cells also retained their MSC-specific cell surface markers. A similar effect was observed on bone and marrow explants, which was further confirmed with confocal microscopy analysis. Moreover, markedly higher bone marrow preservation was observed with histology in case of HAS supplementation compared to FCS. These findings indicate possible application of HAS in regenerative solutions of skeletal diseases like OA or osteonecrosis.
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spelling pubmed-59264802018-05-14 The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche Simon, Melinda Major, Bálint Vácz, Gabriella Kuten, Olga Hornyák, István Hinsenkamp, Adél Kardos, Dorottya Bagó, Marcell Cseh, Domonkos Sárközi, Adrienn Horvathy, Denes Nehrer, Stefan Lacza, Zsombor Stem Cells Int Research Article Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as “proliferative agents” for the topical MSC pool. Hyperacute serum (HAS), a new serum derivative, has been designed to approximate the natural coagulation cascade with a single-step, additive-free preparation method. We investigated the effects of HAS on monolayer MSC cultures and in their natural niche, in 3D subchondral bone and marrow explants. Viability measurements, RT-qPCR evaluation for gene expression and flow cytometry for cell surface marker analysis were performed to compare the effects of FCS-, PRP-, or HAS-supplemented culture media. Monolayer MSCs showed significantly higher metabolic activity following 5 days' incubation in HAS, and osteoblast-specific mRNA expression was markedly increased, while cells also retained their MSC-specific cell surface markers. A similar effect was observed on bone and marrow explants, which was further confirmed with confocal microscopy analysis. Moreover, markedly higher bone marrow preservation was observed with histology in case of HAS supplementation compared to FCS. These findings indicate possible application of HAS in regenerative solutions of skeletal diseases like OA or osteonecrosis. Hindawi 2018-04-16 /pmc/articles/PMC5926480/ /pubmed/29760725 http://dx.doi.org/10.1155/2018/4854619 Text en Copyright © 2018 Melinda Simon et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Simon, Melinda
Major, Bálint
Vácz, Gabriella
Kuten, Olga
Hornyák, István
Hinsenkamp, Adél
Kardos, Dorottya
Bagó, Marcell
Cseh, Domonkos
Sárközi, Adrienn
Horvathy, Denes
Nehrer, Stefan
Lacza, Zsombor
The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
title The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
title_full The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
title_fullStr The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
title_full_unstemmed The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
title_short The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
title_sort effects of hyperacute serum on the elements of the human subchondral bone marrow niche
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5926480/
https://www.ncbi.nlm.nih.gov/pubmed/29760725
http://dx.doi.org/10.1155/2018/4854619
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