A novel assay for the introduction of the vinyl ether double bond into plasmalogens using pyrene-labeled substrates

Plasmanylethanolamine desaturase (PEDS) (EC 1.14.99.19) introduces the 1-prime double bond into plasmalogens, one of the most abundant phospholipids in the human body. This labile membrane enzyme has not been purified and its coding sequence is unknown. Previous assays for this enzyme used radiolabe...

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Detalles Bibliográficos
Autores principales: Werner, Ernst R., Keller, Markus A., Sailer, Sabrina, Seppi, Daniele, Golderer, Georg, Werner-Felmayer, Gabriele, Zoeller, Raphael A., Watschinger, Katrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2018
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928432/
https://www.ncbi.nlm.nih.gov/pubmed/29540573
http://dx.doi.org/10.1194/jlr.D080283

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928432/
https://www.ncbi.nlm.nih.gov/pubmed/29540573
http://dx.doi.org/10.1194/jlr.D080283

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