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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Reporter enzyme fluorescence (REF) utilizes substrates that are specific for enzymes present in target organisms of interest for imaging or detection by fluorescence or bioluminescence. We utilize BlaC, an enzyme expressed constitutively by all M. tuberculosis strains. REF allows rapid quantificatio...

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Detalles Bibliográficos
Autores principales: Sharan, Riti, Yang, Hee-Jeong, Sule, Preeti, Cirillo, Jeffrey D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5931367/
https://www.ncbi.nlm.nih.gov/pubmed/29553533
http://dx.doi.org/10.3791/56801