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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Detergents are indispensable for delivery of membrane proteins into 30-100 nm small unilamellar vesicles, while more complex, larger model lipid bilayers are less compatible with detergents. Here we describe a strategy for bypassing this fundamental limitation using fusogenic oppositely charged lipo...

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Detalles Bibliográficos
Autores principales: Galkin, Mikhail A., Russell, Aidan N., Vik, Steven B., Berry, Richard M., Ishmukhametov, Robert R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933413/
https://www.ncbi.nlm.nih.gov/pubmed/29683454
http://dx.doi.org/10.3791/56909