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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
Detergents are indispensable for delivery of membrane proteins into 30-100 nm small unilamellar vesicles, while more complex, larger model lipid bilayers are less compatible with detergents. Here we describe a strategy for bypassing this fundamental limitation using fusogenic oppositely charged lipo...
Autores principales: | Galkin, Mikhail A., Russell, Aidan N., Vik, Steven B., Berry, Richard M., Ishmukhametov, Robert R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933413/ https://www.ncbi.nlm.nih.gov/pubmed/29683454 http://dx.doi.org/10.3791/56909 |
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