An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However, current protocols for electroporation either require the investment in specialized electroporators or corrosive pre-treatment of zygotes...

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Detalles Bibliográficos
Autores principales: Tröder, Simon E., Ebert, Lena K., Butt, Linus, Assenmacher, Sonja, Schermer, Bernhard, Zevnik, Branko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933690/
https://www.ncbi.nlm.nih.gov/pubmed/29723268
http://dx.doi.org/10.1371/journal.pone.0196891

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5933690/
https://www.ncbi.nlm.nih.gov/pubmed/29723268
http://dx.doi.org/10.1371/journal.pone.0196891

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