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Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis
Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues. Several methods for multiple site-directed mutagenesis exist, but there remains a need for fast and simple methods to ef...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5934624/ https://www.ncbi.nlm.nih.gov/pubmed/29409059 http://dx.doi.org/10.1093/nar/gky067 |