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Selection and evaluation of housekeeping genes as endogenous controls for quantification of mRNA transcripts in Theileria parva using quantitative real-time polymerase chain reaction (qPCR)

The reliability of any quantitative real-time polymerase chain reaction (qPCR) experiment can be seriously compromised by variations between samples as well as between PCR runs. This usually result from errors in sample quantification, especially with samples that are obtained from different individ...

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Detalles Bibliográficos
Autores principales: Tsotetsi, Teboho N., Collins, Nicola E., Oosthuizen, Marinda C., Sibeko-Matjila, Kgomotso P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935388/
https://www.ncbi.nlm.nih.gov/pubmed/29727459
http://dx.doi.org/10.1371/journal.pone.0196715