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Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing
BACKGROUND: Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells. Such amounts are not always attainable from samples having a relatively low DNA or RNA input; or those for which a lim...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935984/ https://www.ncbi.nlm.nih.gov/pubmed/29728062 http://dx.doi.org/10.1186/s12864-018-4677-y |