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Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

BACKGROUND: Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells. Such amounts are not always attainable from samples having a relatively low DNA or RNA input; or those for which a lim...

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Detalles Bibliográficos
Autores principales:	Wu, Wells W., Phue, Je-Nie, Lee, Chun-Ting, Lin, Changyi, Xu, Lai, Wang, Rong, Zhang, Yaqin, Shen, Rong-Fong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5935984/ https://www.ncbi.nlm.nih.gov/pubmed/29728062 http://dx.doi.org/10.1186/s12864-018-4677-y

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