An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation

Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH). Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-ty...

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Detalles Bibliográficos
Autores principales: Dobrzycki, Tomasz, Krecsmarik, Monika, Bonkhofer, Florian, Patient, Roger, Monteiro, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Methods & Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936060/
https://www.ncbi.nlm.nih.gov/pubmed/29535102
http://dx.doi.org/10.1242/bio.031096

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936060/
https://www.ncbi.nlm.nih.gov/pubmed/29535102
http://dx.doi.org/10.1242/bio.031096

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