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An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation
Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines. A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH). Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-ty...
Autores principales: | Dobrzycki, Tomasz, Krecsmarik, Monika, Bonkhofer, Florian, Patient, Roger, Monteiro, Rui |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5936060/ https://www.ncbi.nlm.nih.gov/pubmed/29535102 http://dx.doi.org/10.1242/bio.031096 |
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