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Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937782/ https://www.ncbi.nlm.nih.gov/pubmed/29734364 http://dx.doi.org/10.1371/journal.pone.0196996 |
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author | Huang, Li-Hua Yuan, Ming-Quan Ao, Xiu-Jin Ren, An-Yun Zhang, Han-Bo Yang, Ming-Zhi |
author_facet | Huang, Li-Hua Yuan, Ming-Quan Ao, Xiu-Jin Ren, An-Yun Zhang, Han-Bo Yang, Ming-Zhi |
author_sort | Huang, Li-Hua |
collection | PubMed |
description | Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells. In response to different EFS, quantities of detected metabolites in grape cells varied from 6 to 17 in this assay, and 1 to 11 novel metabolites were introduced into metabolome of grape cells. Dual-culture with fungal strains CS2, RH16 and RH5 introduced the highest quantities (10 or 11) of novel metabolites in grape cells. More importantly, the modification of metabolic profiles in grape cells via fungal endophytes appeared to be fungal strain/genus-specificity. Overall, this work revealed that introduction of specific metabolites in host plants may be one consequence during the process of endophytes-host metabolic interactions, which raise the possibility to shape grape qualities and characteristics using tool of fungal endophytes. |
format | Online Article Text |
id | pubmed-5937782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59377822018-05-18 Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro Huang, Li-Hua Yuan, Ming-Quan Ao, Xiu-Jin Ren, An-Yun Zhang, Han-Bo Yang, Ming-Zhi PLoS One Research Article Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells. In response to different EFS, quantities of detected metabolites in grape cells varied from 6 to 17 in this assay, and 1 to 11 novel metabolites were introduced into metabolome of grape cells. Dual-culture with fungal strains CS2, RH16 and RH5 introduced the highest quantities (10 or 11) of novel metabolites in grape cells. More importantly, the modification of metabolic profiles in grape cells via fungal endophytes appeared to be fungal strain/genus-specificity. Overall, this work revealed that introduction of specific metabolites in host plants may be one consequence during the process of endophytes-host metabolic interactions, which raise the possibility to shape grape qualities and characteristics using tool of fungal endophytes. Public Library of Science 2018-05-07 /pmc/articles/PMC5937782/ /pubmed/29734364 http://dx.doi.org/10.1371/journal.pone.0196996 Text en © 2018 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Huang, Li-Hua Yuan, Ming-Quan Ao, Xiu-Jin Ren, An-Yun Zhang, Han-Bo Yang, Ming-Zhi Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
title | Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
title_full | Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
title_fullStr | Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
title_full_unstemmed | Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
title_short | Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
title_sort | endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937782/ https://www.ncbi.nlm.nih.gov/pubmed/29734364 http://dx.doi.org/10.1371/journal.pone.0196996 |
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