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Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro

Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work,...

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Autores principales: Huang, Li-Hua, Yuan, Ming-Quan, Ao, Xiu-Jin, Ren, An-Yun, Zhang, Han-Bo, Yang, Ming-Zhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937782/
https://www.ncbi.nlm.nih.gov/pubmed/29734364
http://dx.doi.org/10.1371/journal.pone.0196996
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author Huang, Li-Hua
Yuan, Ming-Quan
Ao, Xiu-Jin
Ren, An-Yun
Zhang, Han-Bo
Yang, Ming-Zhi
author_facet Huang, Li-Hua
Yuan, Ming-Quan
Ao, Xiu-Jin
Ren, An-Yun
Zhang, Han-Bo
Yang, Ming-Zhi
author_sort Huang, Li-Hua
collection PubMed
description Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells. In response to different EFS, quantities of detected metabolites in grape cells varied from 6 to 17 in this assay, and 1 to 11 novel metabolites were introduced into metabolome of grape cells. Dual-culture with fungal strains CS2, RH16 and RH5 introduced the highest quantities (10 or 11) of novel metabolites in grape cells. More importantly, the modification of metabolic profiles in grape cells via fungal endophytes appeared to be fungal strain/genus-specificity. Overall, this work revealed that introduction of specific metabolites in host plants may be one consequence during the process of endophytes-host metabolic interactions, which raise the possibility to shape grape qualities and characteristics using tool of fungal endophytes.
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spelling pubmed-59377822018-05-18 Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro Huang, Li-Hua Yuan, Ming-Quan Ao, Xiu-Jin Ren, An-Yun Zhang, Han-Bo Yang, Ming-Zhi PLoS One Research Article Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells. In response to different EFS, quantities of detected metabolites in grape cells varied from 6 to 17 in this assay, and 1 to 11 novel metabolites were introduced into metabolome of grape cells. Dual-culture with fungal strains CS2, RH16 and RH5 introduced the highest quantities (10 or 11) of novel metabolites in grape cells. More importantly, the modification of metabolic profiles in grape cells via fungal endophytes appeared to be fungal strain/genus-specificity. Overall, this work revealed that introduction of specific metabolites in host plants may be one consequence during the process of endophytes-host metabolic interactions, which raise the possibility to shape grape qualities and characteristics using tool of fungal endophytes. Public Library of Science 2018-05-07 /pmc/articles/PMC5937782/ /pubmed/29734364 http://dx.doi.org/10.1371/journal.pone.0196996 Text en © 2018 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Huang, Li-Hua
Yuan, Ming-Quan
Ao, Xiu-Jin
Ren, An-Yun
Zhang, Han-Bo
Yang, Ming-Zhi
Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
title Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
title_full Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
title_fullStr Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
title_full_unstemmed Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
title_short Endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
title_sort endophytic fungi specifically introduce novel metabolites into grape flesh cells in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937782/
https://www.ncbi.nlm.nih.gov/pubmed/29734364
http://dx.doi.org/10.1371/journal.pone.0196996
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