Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency

The CRISPR/Cas9 system is widely applied in genome engineering due to its simplicity and versatility. Although this has revolutionized genome-editing technology, knockin animal generation via homology directed repair (HDR) is not as efficient as nonhomologous end-joining DNA-repair-dependent knockou...

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Detalles Bibliográficos
Autores principales: Jang, Da Eun, Lee, Jae Young, Lee, Jae Hoon, Koo, Ok Jae, Bae, Hee Sook, Jung, Min Hee, Bae, Ji Hyun, Hwang, Woo Sung, Chang, Yoo Jin, Lee, Yoon Hoo, Lee, Han Woong, Yeom, Su Cheong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938013/
https://www.ncbi.nlm.nih.gov/pubmed/29622782
http://dx.doi.org/10.1038/s12276-018-0037-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938013/
https://www.ncbi.nlm.nih.gov/pubmed/29622782
http://dx.doi.org/10.1038/s12276-018-0037-x

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