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A novel α-galactosidase from the thermophilic probiotic Bacillus coagulans with remarkable protease-resistance and high hydrolytic activity

A novel α-galactosidase of glycoside hydrolase family 36 was cloned from Bacillus coagulans, overexpressed in Escherichia coli, and characterized. The purified enzyme Aga-BC7050 was 85 kDa according to SDS-PAGE and 168 kDa according to gel filtration, indicating that its native structure is a dimer....

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Detalles Bibliográficos
Autores principales: Zhao, Ruili, Zhao, Rui, Tu, Yishuai, Zhang, Xiaoming, Deng, Liping, Chen, Xiangdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940202/
https://www.ncbi.nlm.nih.gov/pubmed/29738566
http://dx.doi.org/10.1371/journal.pone.0197067