Locus specific engineering of tandem DNA repeats in the genome of Saccharomyces cerevisiae using CRISPR/Cas9 and overlapping oligonucleotides

DNA repeats constitute a large part of genomes of multicellular eucaryotes. For a longtime considered as junk DNA, their role in genome organization and tuning of gene expression is being increasingly documented. Synthetic biology has so far largely ignored DNA repeats as regulatory elements to mani...

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Detalles Bibliográficos
Autores principales: Lancrey, Astrid, Joubert, Alexandra, Boulé, Jean-Baptiste
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940919/
https://www.ncbi.nlm.nih.gov/pubmed/29740109
http://dx.doi.org/10.1038/s41598-018-25508-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5940919/
https://www.ncbi.nlm.nih.gov/pubmed/29740109
http://dx.doi.org/10.1038/s41598-018-25508-3

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