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Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0

OBJECTIVE: In the human body pathogenic mycobacteria encounter low pH within the phagosomes of macrophages where they reside after being internalized by the host cell. Low pH within macrophages has been shown to induce expression of a variety of genes within these bacteria. It had been previously ob...

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Autores principales: Torres, Rachelle, Dorriz, Donna, Saviola, Beatrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941605/
https://www.ncbi.nlm.nih.gov/pubmed/29739437
http://dx.doi.org/10.1186/s13104-018-3370-1
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author Torres, Rachelle
Dorriz, Donna
Saviola, Beatrice
author_facet Torres, Rachelle
Dorriz, Donna
Saviola, Beatrice
author_sort Torres, Rachelle
collection PubMed
description OBJECTIVE: In the human body pathogenic mycobacteria encounter low pH within the phagosomes of macrophages where they reside after being internalized by the host cell. Low pH within macrophages has been shown to induce expression of a variety of genes within these bacteria. It had been previously observed that the Mycobacterium tuberculosis lipF promoter is transcriptionally upregulated between pHs 4.5–6.4 in Mycobacterium smegmatis, with an upper pH limit of 6.4 capable of promoter induction. To better understand the parameters of acid induced gene expression, we sought to determine the lower pH limit capable of lipF promoter induction. RESULTS: As we had already determined an upper pH limit, we determine here that there is a lower limit of pH’s capable of upregulating the lipF promoter, with pH below 4.3 not positively upregulating the promoter. At non-inducing pH 4.2 the bacterial cells remain viable in the absence of acid induced lipF promoter upregulation and subsequent exposure to acid pH 5.0 results in lipF promoter upregulation. There appears to be a lower limit of pH capable of upregulating lipF promoter expression and this limit is not due to cell death.
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spelling pubmed-59416052018-05-14 Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0 Torres, Rachelle Dorriz, Donna Saviola, Beatrice BMC Res Notes Research Note OBJECTIVE: In the human body pathogenic mycobacteria encounter low pH within the phagosomes of macrophages where they reside after being internalized by the host cell. Low pH within macrophages has been shown to induce expression of a variety of genes within these bacteria. It had been previously observed that the Mycobacterium tuberculosis lipF promoter is transcriptionally upregulated between pHs 4.5–6.4 in Mycobacterium smegmatis, with an upper pH limit of 6.4 capable of promoter induction. To better understand the parameters of acid induced gene expression, we sought to determine the lower pH limit capable of lipF promoter induction. RESULTS: As we had already determined an upper pH limit, we determine here that there is a lower limit of pH’s capable of upregulating the lipF promoter, with pH below 4.3 not positively upregulating the promoter. At non-inducing pH 4.2 the bacterial cells remain viable in the absence of acid induced lipF promoter upregulation and subsequent exposure to acid pH 5.0 results in lipF promoter upregulation. There appears to be a lower limit of pH capable of upregulating lipF promoter expression and this limit is not due to cell death. BioMed Central 2018-05-08 /pmc/articles/PMC5941605/ /pubmed/29739437 http://dx.doi.org/10.1186/s13104-018-3370-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Torres, Rachelle
Dorriz, Donna
Saviola, Beatrice
Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0
title Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0
title_full Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0
title_fullStr Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0
title_full_unstemmed Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0
title_short Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0
title_sort induction of the acid inducible lipf promoter is reversibly inhibited in ph ranges of ph 4.2-4.0
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941605/
https://www.ncbi.nlm.nih.gov/pubmed/29739437
http://dx.doi.org/10.1186/s13104-018-3370-1
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