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Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask
BACKGROUND: Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism. As a screening tool on shake flask level, the membrane-based fed-batch shake...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941677/ https://www.ncbi.nlm.nih.gov/pubmed/29743073 http://dx.doi.org/10.1186/s12934-018-0917-8 |