Stepwise partially overlapping primer-based PCR for genome walking

A stepwise partially overlapping primer-based PCR (SWPOP-PCR) method for isolating flanking unknown DNA regions was developed, which comprises three rounds of nested PCRs sequentially driven by SWPOP primer-nested specific primer pairs. SWPOP primer set is characterized by a partial overlap of 10 bp...

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Detalles Bibliográficos
Autores principales: Chang, Kunpeng, Wang, Qiong, Shi, Xiaofei, Wang, Shuixing, Wu, Hongjing, Nie, Lijuan, Li, Haixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943200/
https://www.ncbi.nlm.nih.gov/pubmed/29744607
http://dx.doi.org/10.1186/s13568-018-0610-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943200/
https://www.ncbi.nlm.nih.gov/pubmed/29744607
http://dx.doi.org/10.1186/s13568-018-0610-7

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