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A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture

Air-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover, stringent breeding schem...

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Detalles Bibliográficos
Autores principales: Eenjes, Evelien, Mertens, Tinne C. J., Buscop-van Kempen, Marjon J., van Wijck, Yolanda, Taube, Christian, Rottier, Robbert J., Hiemstra, Pieter S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943313/
https://www.ncbi.nlm.nih.gov/pubmed/29743551
http://dx.doi.org/10.1038/s41598-018-25799-6