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A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture
Air-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover, stringent breeding schem...
Autores principales: | Eenjes, Evelien, Mertens, Tinne C. J., Buscop-van Kempen, Marjon J., van Wijck, Yolanda, Taube, Christian, Rottier, Robbert J., Hiemstra, Pieter S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5943313/ https://www.ncbi.nlm.nih.gov/pubmed/29743551 http://dx.doi.org/10.1038/s41598-018-25799-6 |
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