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Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy

[Image: see text] We present a simple and versatile single-molecule-based method for the accurate determination of binding rates to surfaces or surface bound receptors. To quantify the reversible surface attachment of fluorescently labeled molecules, we have modified previous schemes for fluorescenc...

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Detalles Bibliográficos
Autores principales: Mücksch, Jonas, Blumhardt, Philipp, Strauss, Maximilian T., Petrov, Eugene P., Jungmann, Ralf, Schwille, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946168/
https://www.ncbi.nlm.nih.gov/pubmed/29658275
http://dx.doi.org/10.1021/acs.nanolett.8b00875