An Efficient System for Gene Perturbation in Embryonic Hippocampal Progenitors Using Ex Vivo Electroporation Followed by In Vitro Dissociated Cell Culture

We established an efficient cell culture assay that permits combinatorial genetic perturbations in hippocampal progenitors to examine cell-autonomous mechanisms of fate specification. The procedure begins with ex vivo electroporation of isolated, intact embryonic brains, in a manner similar to in ut...

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Detalles Bibliográficos
Autores principales: Muralidharan, Bhavana, D’Souza, Leora, Tole, Shubha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946340/
https://www.ncbi.nlm.nih.gov/pubmed/29760561
http://dx.doi.org/10.1177/1179069518767404

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5946340/
https://www.ncbi.nlm.nih.gov/pubmed/29760561
http://dx.doi.org/10.1177/1179069518767404

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