A microfluidic chip with a staircase pH gradient generator, a packed column and a fraction collector for chromatofocusing of proteins

A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro‐column, pH gradient generation, and fraction collection in a single device. Using a sieve micro‐valve, anion exchange particles were packed into a microchannel in order to realize a solid‐phase ab...

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Detalles Bibliográficos
Autores principales: Rho, Hoon Suk, Hanke, Alexander Thomas, Ottens, Marcel, Gardeniers, Han J.G.E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Part I. Proteins and Proteomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947739/
https://www.ncbi.nlm.nih.gov/pubmed/29345313
http://dx.doi.org/10.1002/elps.201700341

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947739/
https://www.ncbi.nlm.nih.gov/pubmed/29345313
http://dx.doi.org/10.1002/elps.201700341

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