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A microfluidic chip with a staircase pH gradient generator, a packed column and a fraction collector for chromatofocusing of proteins
A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro‐column, pH gradient generation, and fraction collection in a single device. Using a sieve micro‐valve, anion exchange particles were packed into a microchannel in order to realize a solid‐phase ab...
Autores principales: | Rho, Hoon Suk, Hanke, Alexander Thomas, Ottens, Marcel, Gardeniers, Han J.G.E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5947739/ https://www.ncbi.nlm.nih.gov/pubmed/29345313 http://dx.doi.org/10.1002/elps.201700341 |
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