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Randomized DNA libraries construction tool: a new 3-bp ‘frequent cutter’ TthHB27I/sinefungin endonuclease with chemically-induced specificity

BACKGROUND: Acoustic or hydrodynamic shearing, sonication and enzymatic digestion are used to fragment DNA. However, these methods have several disadvantages, such as DNA damage, difficulties in fragmentation control, irreproducibility and under-representation of some DNA segments. The DNA fragmenta...

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Detalles Bibliográficos
Autores principales: Krefft, Daria, Papkov, Aliaksei, Prusinowski, Maciej, Zylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948728/
https://www.ncbi.nlm.nih.gov/pubmed/29751745
http://dx.doi.org/10.1186/s12864-018-4748-0