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Randomized DNA libraries construction tool: a new 3-bp ‘frequent cutter’ TthHB27I/sinefungin endonuclease with chemically-induced specificity

BACKGROUND: Acoustic or hydrodynamic shearing, sonication and enzymatic digestion are used to fragment DNA. However, these methods have several disadvantages, such as DNA damage, difficulties in fragmentation control, irreproducibility and under-representation of some DNA segments. The DNA fragmenta...

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Detalles Bibliográficos
Autores principales:	Krefft, Daria, Papkov, Aliaksei, Prusinowski, Maciej, Zylicz-Stachula, Agnieszka, Skowron, Piotr M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948728/ https://www.ncbi.nlm.nih.gov/pubmed/29751745 http://dx.doi.org/10.1186/s12864-018-4748-0

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