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Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media

BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply wit...

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Detalles Bibliográficos
Autores principales: Agostini, Francesco, Rossi, Francesca Maria, Aldinucci, Donatella, Battiston, Monica, Lombardi, Elisabetta, Zanolin, Stefania, Massarut, Samuele, Parodi, Pier Camillo, Da Ponte, Alessandro, Tessitori, Giovanni, Pivetta, Barbara, Durante, Cristina, Mazzucato, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948766/
https://www.ncbi.nlm.nih.gov/pubmed/29751821
http://dx.doi.org/10.1186/s13287-018-0886-1