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Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria

Native cell-free transcription–translation systems offer a rapid route to characterize the regulatory elements (promoters, transcription factors) for gene expression from nonmodel microbial hosts, which can be difficult to assess through traditional in vivo approaches. One such host, Bacillus megate...

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Detalles Bibliográficos
Autores principales: Moore, Simon J., MacDonald, James T., Wienecke, Sarah, Ishwarbhai, Alka, Tsipa, Argyro, Aw, Rochelle, Kylilis, Nicolas, Bell, David J., McClymont, David W., Jensen, Kirsten, Polizzi, Karen M., Biedendieck, Rebekka, Freemont, Paul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948957/
https://www.ncbi.nlm.nih.gov/pubmed/29666238
http://dx.doi.org/10.1073/pnas.1715806115