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Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
Native cell-free transcription–translation systems offer a rapid route to characterize the regulatory elements (promoters, transcription factors) for gene expression from nonmodel microbial hosts, which can be difficult to assess through traditional in vivo approaches. One such host, Bacillus megate...
Autores principales: | Moore, Simon J., MacDonald, James T., Wienecke, Sarah, Ishwarbhai, Alka, Tsipa, Argyro, Aw, Rochelle, Kylilis, Nicolas, Bell, David J., McClymont, David W., Jensen, Kirsten, Polizzi, Karen M., Biedendieck, Rebekka, Freemont, Paul S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948957/ https://www.ncbi.nlm.nih.gov/pubmed/29666238 http://dx.doi.org/10.1073/pnas.1715806115 |
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