Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II

Initiation and promoter-proximal pausing are key regulatory steps of RNA Polymerase II (Pol II) transcription. To study the in vivo dynamics of endogenous Pol II during these steps, we generated fully functional GFP-RPB1 knockin cells. GFP-RPB1 photobleaching combined with computational modeling rev...

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Detalles Bibliográficos
Autores principales: Steurer, Barbara, Janssens, Roel C., Geverts, Bart, Geijer, Marit E., Wienholz, Franziska, Theil, Arjan F., Chang, Jiang, Dealy, Shannon, Pothof, Joris, van Cappellen, Wiggert A., Houtsmuller, Adriaan B., Marteijn, Jurgen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
PNAS Plus
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948963/
https://www.ncbi.nlm.nih.gov/pubmed/29632207
http://dx.doi.org/10.1073/pnas.1717920115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5948963/
https://www.ncbi.nlm.nih.gov/pubmed/29632207
http://dx.doi.org/10.1073/pnas.1717920115

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