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Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars

In an effort to produce reliably contained transgenic trees, we used the CRISPR/Cas9 system to alter three genes expected to be required for normal flowering in poplar (genus Populus). We designed synthetic guide RNAs (sgRNAs) to target the poplar homolog of the floral meristem identity gene, LEAFY...

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Detalles Bibliográficos
Autores principales: Elorriaga, Estefania, Klocko, Amy L., Ma, Cathleen, Strauss, Steven H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5949366/
https://www.ncbi.nlm.nih.gov/pubmed/29868058
http://dx.doi.org/10.3389/fpls.2018.00594