A multi-phenotypic imaging screen to identify bacterial effectors by exogenous expression in a HeLa cell line

We present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa cells expressing an autophagy reporter (mcherry-LC3) and one of 224 GFP-fused proteins from the Crohn’s Disease (CD)-associated bacterium, Adherent Invasive E. coli (AIEC) strain LF82. Using automated...

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Detalles Bibliográficos
Autores principales: Collins, Adam, Huett, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2018
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952863/
https://www.ncbi.nlm.nih.gov/pubmed/29762554
http://dx.doi.org/10.1038/sdata.2018.81

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952863/
https://www.ncbi.nlm.nih.gov/pubmed/29762554
http://dx.doi.org/10.1038/sdata.2018.81

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