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A multi-phenotypic imaging screen to identify bacterial effectors by exogenous expression in a HeLa cell line
We present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa cells expressing an autophagy reporter (mcherry-LC3) and one of 224 GFP-fused proteins from the Crohn’s Disease (CD)-associated bacterium, Adherent Invasive E. coli (AIEC) strain LF82. Using automated...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5952863/ https://www.ncbi.nlm.nih.gov/pubmed/29762554 http://dx.doi.org/10.1038/sdata.2018.81 |