A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to decipher gene functions and genetic interactions. Here we use a genome-wide library of guide RNAs to direct the catalytically dead Cas9 (dCas9) to block gene transcription in Escherichia coli. Using a machine-learning app...

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Detalles Bibliográficos
Autores principales: Cui, Lun, Vigouroux, Antoine, Rousset, François, Varet, Hugo, Khanna, Varun, Bikard, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954155/
https://www.ncbi.nlm.nih.gov/pubmed/29765036
http://dx.doi.org/10.1038/s41467-018-04209-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5954155/
https://www.ncbi.nlm.nih.gov/pubmed/29765036
http://dx.doi.org/10.1038/s41467-018-04209-5

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