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Expressed repetitive elements are broadly applicable reference targets for normalization of reverse transcription-qPCR data in mice

Reverse transcription quantitative PCR (RT-qPCR) is the gold standard method for gene expression analysis on mRNA level. To remove experimental variation, expression levels of the gene of interest are typically normalized to the expression level of stably expressed endogenous reference genes. Identi...

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Detalles Bibliográficos
Autores principales: Renard, Marjolijn, Vanhauwaert, Suzanne, Vanhomwegen, Marine, Rihani, Ali, Vandamme, Niels, Goossens, Steven, Berx, Geert, Van Vlierberghe, Pieter, Haigh, Jody J., Decaesteker, Bieke, Van Laere, Jolien, Lambertz, Irina, Speleman, Frank, Vandesompele, Jo, Willaert, Andy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5955877/
https://www.ncbi.nlm.nih.gov/pubmed/29769563
http://dx.doi.org/10.1038/s41598-018-25389-6