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Methanol fixed fibroblasts serve as feeder cells to maintain stem cells in the pluripotent state in vitro

Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we demonstrated a novel method to prepare feeder cells with high efficiency, timesaving, and low costs. MEFs in 3 × 10(4) cell/cm(2) were fixe...

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Detalles Bibliográficos
Autores principales: Ren, Yahui, Ma, Ziyu, Yu, Tong, Ling, Min, Wang, Huayan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958091/
https://www.ncbi.nlm.nih.gov/pubmed/29773904
http://dx.doi.org/10.1038/s41598-018-26238-2