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Methanol fixed fibroblasts serve as feeder cells to maintain stem cells in the pluripotent state in vitro
Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we demonstrated a novel method to prepare feeder cells with high efficiency, timesaving, and low costs. MEFs in 3 × 10(4) cell/cm(2) were fixe...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958091/ https://www.ncbi.nlm.nih.gov/pubmed/29773904 http://dx.doi.org/10.1038/s41598-018-26238-2 |