High-throughput, image-based screening of pooled genetic variant libraries

Image-based, high-throughput screening of genetic perturbations will advance both biology and biotechnology. We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in numerous individual cells. We achieve genotyping by introducing barcode...

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Detalles Bibliográficos
Autores principales: Emanuel, George, Moffitt, Jeffrey R., Zhuang, Xiaowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958624/
https://www.ncbi.nlm.nih.gov/pubmed/29083401
http://dx.doi.org/10.1038/nmeth.4495
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author Emanuel, George
Moffitt, Jeffrey R.
Zhuang, Xiaowei
author_facet Emanuel, George
Moffitt, Jeffrey R.
Zhuang, Xiaowei
author_sort Emanuel, George
collection PubMed
description Image-based, high-throughput screening of genetic perturbations will advance both biology and biotechnology. We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in numerous individual cells. We achieve genotyping by introducing barcoded genetic variants into cells and using massively multiplexed FISH to measure the barcodes. We demonstrated this method by screening mutants of the fluorescent protein YFAST, yielding brighter and more photostable YFAST variants.
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spelling pubmed-59586242018-05-18 High-throughput, image-based screening of pooled genetic variant libraries Emanuel, George Moffitt, Jeffrey R. Zhuang, Xiaowei Nat Methods Article Image-based, high-throughput screening of genetic perturbations will advance both biology and biotechnology. We report a high-throughput screening method that allows diverse genotypes and corresponding phenotypes to be imaged in numerous individual cells. We achieve genotyping by introducing barcoded genetic variants into cells and using massively multiplexed FISH to measure the barcodes. We demonstrated this method by screening mutants of the fluorescent protein YFAST, yielding brighter and more photostable YFAST variants. 2017-10-30 2017-12 /pmc/articles/PMC5958624/ /pubmed/29083401 http://dx.doi.org/10.1038/nmeth.4495 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Emanuel, George
Moffitt, Jeffrey R.
Zhuang, Xiaowei
High-throughput, image-based screening of pooled genetic variant libraries
title High-throughput, image-based screening of pooled genetic variant libraries
title_full High-throughput, image-based screening of pooled genetic variant libraries
title_fullStr High-throughput, image-based screening of pooled genetic variant libraries
title_full_unstemmed High-throughput, image-based screening of pooled genetic variant libraries
title_short High-throughput, image-based screening of pooled genetic variant libraries
title_sort high-throughput, image-based screening of pooled genetic variant libraries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5958624/
https://www.ncbi.nlm.nih.gov/pubmed/29083401
http://dx.doi.org/10.1038/nmeth.4495
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AT zhuangxiaowei highthroughputimagebasedscreeningofpooledgeneticvariantlibraries

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