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Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France
INTRODUCTION: A second botulism outbreak due to Clostridium baratii occurred in France in August 2015 and included three patients who had their meal in a restaurant the same day. We report the characterization of C. baratii isolates including whole genome sequencing (WGS). METHODS: Four C. baratii i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5959735/ https://www.ncbi.nlm.nih.gov/pubmed/29862134 http://dx.doi.org/10.1371/currents.outbreaks.6ed2fe754b58a5c42d0c33d586ffc606 |
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author | Mazuet, Christelle Legeay, Christine Sautereau, Jean Bouchier, Christiane Criscuolo, Alexis Bouvet, Philippe Trehard, Hélène Jourdan Da Silva, Nathalie Popoff, Michel |
author_facet | Mazuet, Christelle Legeay, Christine Sautereau, Jean Bouchier, Christiane Criscuolo, Alexis Bouvet, Philippe Trehard, Hélène Jourdan Da Silva, Nathalie Popoff, Michel |
author_sort | Mazuet, Christelle |
collection | PubMed |
description | INTRODUCTION: A second botulism outbreak due to Clostridium baratii occurred in France in August 2015 and included three patients who had their meal in a restaurant the same day. We report the characterization of C. baratii isolates including whole genome sequencing (WGS). METHODS: Four C. baratii isolates collected in August 2015 from the outbreak 2 were analysed for toxin production and typing as well as for genetic characterization. WGS was done using using the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs) and sequenced on MiSeq machine (Illumina) in paired-end reads of 250 bases. The phylogenetic tree was generated based on the UPGMA method with genetic distances computed by using the Kimura two-parameter model. Evolutionary analyses were conducted in Bionumerics (V.6.6 Applied Maths). RESULTS: Three C. baratii isolates for patient's stools and one isolate from meat produced botulinum neurotoxin (BoNT) type F and retained a bont/F7 gene in OrfX cluster. All isolates were identical according to the WGS. However, phylogeny of the core genome showed that the four C. baratii strains were distantly related to that of the previous C. baratii outbreak in France in 2014 and from the other C. baratii strains reported in databanks. DISCUSSION: The fact that the strains isolated from the patients and meat samples were genetically identical supports that the meat used for the Bolognese sauce was responsible for this second botulism outbreak in France. These isolates were unrelated to that from the first C. baratii outbreak in France in 2014 indicating a distinct source of contamination. WGS provided robust determination of genetic relatedness and information regarding BoNT typing and toxin gene locus genomic localization. |
format | Online Article Text |
id | pubmed-5959735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59597352018-06-01 Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France Mazuet, Christelle Legeay, Christine Sautereau, Jean Bouchier, Christiane Criscuolo, Alexis Bouvet, Philippe Trehard, Hélène Jourdan Da Silva, Nathalie Popoff, Michel PLoS Curr Research Article INTRODUCTION: A second botulism outbreak due to Clostridium baratii occurred in France in August 2015 and included three patients who had their meal in a restaurant the same day. We report the characterization of C. baratii isolates including whole genome sequencing (WGS). METHODS: Four C. baratii isolates collected in August 2015 from the outbreak 2 were analysed for toxin production and typing as well as for genetic characterization. WGS was done using using the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs) and sequenced on MiSeq machine (Illumina) in paired-end reads of 250 bases. The phylogenetic tree was generated based on the UPGMA method with genetic distances computed by using the Kimura two-parameter model. Evolutionary analyses were conducted in Bionumerics (V.6.6 Applied Maths). RESULTS: Three C. baratii isolates for patient's stools and one isolate from meat produced botulinum neurotoxin (BoNT) type F and retained a bont/F7 gene in OrfX cluster. All isolates were identical according to the WGS. However, phylogeny of the core genome showed that the four C. baratii strains were distantly related to that of the previous C. baratii outbreak in France in 2014 and from the other C. baratii strains reported in databanks. DISCUSSION: The fact that the strains isolated from the patients and meat samples were genetically identical supports that the meat used for the Bolognese sauce was responsible for this second botulism outbreak in France. These isolates were unrelated to that from the first C. baratii outbreak in France in 2014 indicating a distinct source of contamination. WGS provided robust determination of genetic relatedness and information regarding BoNT typing and toxin gene locus genomic localization. Public Library of Science 2017-02-01 /pmc/articles/PMC5959735/ /pubmed/29862134 http://dx.doi.org/10.1371/currents.outbreaks.6ed2fe754b58a5c42d0c33d586ffc606 Text en © 2018 Mazuet, Legeay, Sautereau, Bouchier, Criscuolo, Bouvet, Trehard, Jourdan Da Silva, Popoff, et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mazuet, Christelle Legeay, Christine Sautereau, Jean Bouchier, Christiane Criscuolo, Alexis Bouvet, Philippe Trehard, Hélène Jourdan Da Silva, Nathalie Popoff, Michel Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France |
title | Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France |
title_full | Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France |
title_fullStr | Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France |
title_full_unstemmed | Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France |
title_short | Characterization of Clostridium Baratii Type F Strains Responsible for an Outbreak of Botulism Linked to Beef Meat Consumption in France |
title_sort | characterization of clostridium baratii type f strains responsible for an outbreak of botulism linked to beef meat consumption in france |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5959735/ https://www.ncbi.nlm.nih.gov/pubmed/29862134 http://dx.doi.org/10.1371/currents.outbreaks.6ed2fe754b58a5c42d0c33d586ffc606 |
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